Fig. 6

New RM increases natural killer cell viability after transfection. Representative flow cytometry analysis of primary human CD4⁺ cells and natural killer (NK) cells after electroporation with pmax-GFP. Cells were transfected pmax-GFP and cultured for 12 hours at 32 °C either in AIM-V (middle) or our new RM (right). Cell viability was assessed for CD4⁺ cells (A) and NK cells (B) 12 hours post-electroporation using flow cytometry. Viable cells were gated based on forward and side scatter (left). Viable cells were normalized to control (untransfected cells). Numbers in plots represent the percentage of viable cells in the gate. Transfection efficiency was measured for CD4⁺ cells (C) and NK cells (D) using flow cytometry 12 hours after electroporation. Transfection efficiency corresponds to the percentage of GFP-positive cells relative to the percentage of viable cells in each condition. Numbers in plots represent the percentage of GFP-positive cells in the gate