Fig. 3
From: A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
Transfection efficiency of mouse CTLs is maximal in our new recovery medium. A to E. Representative brightfield (left) and corresponding TIRFM (right) images of primary mouse CTLs electroporated with CD81-SEP and cultured as described in Fig. 1. CTLs were seeded on anti-CD3ε coated coverslips to form an immunological synapse. Images were captured 10 min after seeding. Scale bar: 5 μm. Shown are the result of (A) RPMI, (B) Opti-MEM-GlutaMAX, (C) RPMI with Lonza’s complements A and B, (D) Lonza’s complete RM and (E) our new RM. F Quantitative analysis of transfection efficiency expressed as the percentage of fluorescently labeled cells relative to the total number of viable cells. Data represents mean ± SEM. N = 4, *p < 0.05, **p < 0.01, *** p < 0.001