Fig. 1
From: A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
Effect of recovery media on cell viability post-electroporation. A Representative brightfield images of primary mouse CTLs after transfection with CD81-SEP in comparison to non-electroporated control cells. Cells were cultivated post-transfection in indicated recovery media for 12 hours at 32 °C and transferred to AIM-V medium for additional 2 to 6 hours at 37 °C before measurements. Lonza RM corresponds to Lonza’s proprietary media complemented with substances A and B. New RM corresponds to Opti-MEM-GlutaMAX supplemented with 10 mM HEPES, 1% DMSO and 1 mM sodium pyruvate. All media contained 10% FCS. Scale bar: 5 μm. B Quantitative analysis of cell viability 14 hours post-electroporation using indicated recovery media. Cell viability was determined by measuring the percentage of live cells relative to the total number of cells. Data represents mean ± standard error of the mean (SEM). N = 4 independent experiments, *p < 0.05, **p < 0.01, *** p < 0.001