Lot number | CML standard curve series concentration (μmol/L) | |||||||||||
0.025 | 0.050 | 0.100 | 0.300 | 0.450 | 0.700 | 1.200 | 1.500 | LLOQ | Ranges | curve equations | linearity | |
1 | 0.025 | 0.048 | 0.104 | 0.316 | 0.482 | 0.702 | 1.060 | 1.478 | 0.025 | 0.025-1.500 | y = 0.140x-0.000076 | 0.9954 |
2 | 0.024 | 0.056 | 0.097 | 0.291 | 0.475 | 0.738 | 1.087 | 1.461 | 0.024 | 0.025-1.500 | y = 0.137x-0.001250 | 0.9928 |
3 | 0.026 | 0.043 | 0.115 | 0.270 | 0.497 | 0.719 | 1.114 | 1.502 | 0.026 | 0.025-1.500 | y = 0.097x-0.000530 | 0.9862 |
4 | 0.025 | 0.052 | 0.089 | 0.257 | 0.486 | 0.725 | 1.277 | 1.531 | 0.025 | 0.025-1.500 | y = 0.081x-0.000298 | 0.9908 |
5 | 0.026 | 0.045 | 0.099 | 0.285 | 0.501 | 0.775 | 1.164 | 1.382 | 0.026 | 0.025-1.500 | y = 0.180x-0.000395 | 0.9910 |
Mean | 0.025 | 0.049 | 0.101 | 0.284 | 0.488 | 0.732 | 1.140 | 1.471 | 0.025 | |||
SD | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | |||
Relative standard deviation (RSD) (%) | 3.4 | 10.8 | 9.6 | 7.9 | 2.2 | 3.8 | 7.5 | 3.9 | 3.4 | |||
Relative error (RE) (%) | 0.8 | −2.4 | 0.8 | −5.4 | 8.5 | 4.5 | −5.0 | −1.9 | 0.8 | |||
N | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | |||
Lot number | CEL standard curve series concentration (μmol/L) | |||||||||||
0.025 | 0.050 | 0.100 | 0.300 | 0.450 | 0.700 | 1.200 | 1.500 | LLOQ | Ranges | curve equations | linearity | |
1 | 0.025 | 0.048 | 0.099 | 0.315 | 0.506 | 0.724 | 1.064 | 1.414 | 0.025 | 0.025-1.500 | y = 0.624x-0.000541 | 0.9928 |
2 | 0.024 | 0.054 | 0.100 | 0.281 | 0.496 | 0.721 | 1.116 | 1.442 | 0.024 | 0.025-1.500 | y = 0.570x-0.002710 | 0.9946 |
3 | 0.026 | 0.047 | 0.090 | 0.292 | 0.502 | 0.727 | 1.048 | 1.669 | 0.026 | 0.025-1.500 | y = 0.354x-0.001160 | 0.9880 |
4 | 0.026 | 0.048 | 0.091 | 0.277 | 0.486 | 0.728 | 1.246 | 1.496 | 0.025 | 0.025-1.500 | y = 0.316x-0.001310 | 0.9936 |
5 | 0.025 | 0.049 | 0.098 | 0.289 | 0.516 | 0.754 | 1.118 | 1.371 | 0.026 | 0.025-1.500 | y = 0.704x-0.001650 | 0.9918 |
Mean | 0.025 | 0.049 | 0.096 | 0.291 | 0.501 | 0.731 | 1.118 | 1.478 | 0.025 | |||
SD | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.2 | 0.1 | |||
Relative standard deviation (RSD) (%) | 3.4 | 5.7 | 5.0 | 5.1 | 2.3 | 1.9 | 7.0 | 7.9 | 3.4 | |||
Relative error (RE) (%) | 0.8 | −1.6 | −4.4 | −3.1 | 11.4 | 4.4 | −6.8 | −1.4 | 0.8 | |||
N | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |