Skip to main content
Fig. 3 | BMC Biotechnology

Fig. 3

From: Reaping the benefits of liquid handlers for high-throughput gene expression profiling in a marine model invertebrate

Fig. 3

Quality control of RNA extraction and cDNA synthesis using manual and automated procedures. (A and D) Gel electrophoresis of extracted RNA using manual and automated protocol, respectively. (B and E) Gel electrophoresis of PCR amplification of Act coding gene using, as a template, cDNA obtained through the manual and automated procedure, respectively. (C and F) RIN values of RNA extracted using manual (samples A1-7 from picture A) and automated (samples D1-7 from picture D) protocols. The numbers from 1 to 7 indicate sample treated as discussed in method section: 1, Control; 2, Pam2CSK4 30 min; 3, Pam2CSK4 2 h; 4, Pam2CSK4 4 h; 5, zymosan 30 min; 6, zymosan 2 h; 7, zymosan 4 h. Original files of the cropped gels shown in the figure are available in Supplementary Material 5

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us