Table 1 Summary of the purification of L-arginase from marine Bacillus licheniformis OF2
Purification steps | Total arginase activity (U/ml) | Total protein (mg/ml) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude | 8875 ± 0.161 | 362 ± 0.118 | 24.51 ± 0.154 | 1 | 100 |
(NH4)2SO4 40% | 499 ± 0.091 | 4.48 ± 0.089 | 111.383 ± 0.120 | 4.522 | 5.622 |
Sephadex G-100 | 633 ± 0.115 | 2.43 ± 0.201 | 260.49 ± 0.151 | 10.627 | 7.132 |
DEAE-Cellulose | 384.4 ± 0.108 | 0.66 ± 0.096 | 582.42 ± 0.134 | 23.762 | 4.331 |