Fig. 7

a In vitro screening of the antiproliferative activities of L-arginase against human colorectal carcinoma (HCT-116 cell line), human breast carcinoma (MCF-7 cell line), human prostate cancer (PC3 cell line), human melanoma (Mel501 cell line), human pancreatic tumor cell line (Paca2), human lung carcinoma (A-549 cell line), human melanoma (A-375 cell line), human colon cancer (caco2 cell line), human liver carcinoma (HepG2). The preliminary concentration for screening was 100 μg/ml. Each result is a mean of 3 replicate samples and values are represented as % inhibition. b BCL2, BAX, BAX/BCL2 ratio and Casp 7 protein level in MCF-7 cells after treatment with L-arginase for 24 h. c Cellular mechanism of action of L-arginase (A, B) apoptotic induction of L-arginase. MCF-7 was treated with L-arginase for 24 h and analyzed by annexin V/PI staining. The percentage of apoptotic cells is the sum of early apoptotic (annexin V + /PI −) cell percentage and late apoptotic (annexin V + /PI +) cell percentage. (C, D) Cell cycle analysis of MCF-7 after incubation with compound L-arginase for 24 h. Untreated cells were used as a control