Fig. 2
From: Tuning spacer length improves the functionality of the nanobody-based VEGFR2 CAR T cell
Production of IFN-γ (A) and IL-2 (B) as the indicator of CAR T cell activation in response to antigen stimulation. CAR T cells or mock-transduced T cells were co-cultured with either VEGFR2-expressing 293-KDR or VEGFR2− HEK-293 cell lines, and the cytokine level in the co-culture medium was measured using ELISA. The concentrations of IFN-γ and IL-2 were 879 pg/ml and 1924 pg/ml, respectively, in the case of long-spacer CARs and 369 pg/ml and 510 pg/ml for short-spacer VEGFR2-CAR T cells. Results were presented as means ± SD of duplicate assays (n = 3) (***p < 0.001, **p < 0.01)