Fig. 1

Q1Analysis of CAR and VEGFR-2 expression on the cell surface. (A) Scheme of the short and long spacer CARs used in this study and the positioning of their coding segments. (B) Analysis of CAR expression on the surface of T cells from one of the donors transduced with vectors encoding the VEGFR2-CARs with the expression of the short spacer CAR on 52% and long spacer CAR on 54% of the T cells, while undetectable on mock-transduced T cells (light grey histogram). (C) Phenotypic analysis of the T cells seven days post transduction. The CD3 + T cells were 91.4 ± 3.4% of the T cell population and the majority of them (67 ± 5.3%) consisted of CD8 + T cells. The results of Isotype control, along with mock, long spacer and short spacer CAR transduced T cells for on donor have been represented. (D) Studying VEGFR2 presentation on the surface of 293-KDR and HEK-293 cells using polyclonal antibodies. The deep grey histogram shows 293-KDR (90%), and the dotted histogram represents HEK-293