Fig. 5

Significantly up- and downregulated genes in the VB₁₂ synthesis pathway between the S305 strain and the Casida A strain. A Schematic diagram of the VB₁₂ synthesis pathway. The asterisk in the graph represents a significant change in the transcriptional intensity of the gene in strain S305 relative to that in the Casida A strain. The up arrow represents upregulation, and the down arrow represents downregulation. P value < 0.01 is indicated by **. B Relative expression of genes significantly differentially expressed in the VB₁₂ synthesis pathway between the S305 strain and the Casida A strain. The transcription level of the cobP gene was highest at 48 h, with the highest relative expression of other genes occurring at 12 h. The relative expression of genes was calculated using the 2-^△△T relative quantification method