Fig. 2

Basic growth morphology and molecular identification of the S305 strain. A Colony morphology of S305 on solid plates. B Microscopy examination of the S305 strain with Gram staining. The image was obtained at 1000 × magnification (objective oil lens: 100x; eye piece: 10 × magnification). C Phylogenetic evolutionary tree of the S305 16S rRNA constructed using the neighbour joining (NJ) method. The sequences aligned were 16S rRNA (DQ911548.1) from Sinorhizobium sp. AFK-13; 16S rDNA (HQ288937.1) from Sinorhizobium sp. A1 (2011); 16S rRNA (KR819181.1) from Ensifer adhaerens G85; 16S rRNA (JQ927221.1) from Sinorhizobium sp. ZJB1101; 16S rRNA (KC934865.1) from Ensifer adhaerens M28; 16S rRNA (AF227753.1) from Sinorhizobium sp. C4; 16S rRNA (AF285962.1) from Sinorhizobium sp. S002; 16S rRNA (DQ786804.1) from Sinorhizobium fredii SjzZ4; 16S rRNA (AJ505297.1) from Sinorhizobium sp. TJ170; 16S rRNA (JQ322555.1) from Sinorhizobium meliloti CHW10B; 16S rRNA (AJ012210.1) from Sinorhizobium sp. BK1; 16S rRNA (MN083303.1) from Ensifer adhaerens HP1; 16S rRNA (MW800616.1) from Ensifer adhaerens S305; and 16S rRNA (CP015880.1) from Ensifer adhaerens Casida A. The numbers on the branches represents the self-expansion value, the branch clustering and length represent the phylogenetic relationships, and a "T" on the top right of the bacterial species name indicates that the strain is a representative species of the genus. D Phylogenetic evolutionary tree based on the entire genome of S305 and other strains. The genome sequences aligned were as follows: NZ_CP016450.1 from Sinorhizobium sp. RAC02; GCA_000697965.2 from Ensifer adhaerens Casida A; GCA_029439625.1 from Ensifer adhaerens S305; NZ_SSBU01000001.11 from Ensifer adhaerens YX1;GCA_009883655.1 from Ensifer adhaerens Corn53; NZ_CP007236.1 from Ensifer adhaerens OV14; NC_016812.1 from Sinorhizobium fredii HH103; NC_012587.1 from Sinorhizobium fredii NGR234; NZ_CP023063.1 from Sinorhizobium sp. CCBAU 05631; GCA_000006965.1 from Sinorhizobium meliloti 1021; GCA_002197045.1 from Sinorhizobium meliloti RM41; GCA_000218265.1 from Sinorhizobium meliloti SM11; GOLD: Go0003237 from Rhizobium etli CIAT 652; GOLD: Go0000395 from Rhizobium etli CFN 42; GOLD: Go0001809 from Rhizobium leguminosarum bv trifolii WSM1325; NZ_CP004143.1 from Pseudomonas denitrificans ATCC 13867. Branch clustering and length represent phylogenetic relationships, and a "T" on the top right of the strain name indicates that the strain is a representative strain of the genus