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Table 1 sgRNAs and PCR primers used in this study

From: Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

Name

Sequence (5’-3’)

Rosa26-sgRNA

GTGTCGCAATTTCCTGATGA AGG1

H11-sgRNA

TAGGGGACTAATGAAGTACC AGG1

Cep112-sgRNA

ACGCTACATGCATATCCTAC AGG1

Rosa26-PCR-F

AGGGGATTGAGCAGGTGTA

Rosa26-PCR-R

CTAAAACAGGTGAGGAGAAAGC

H11-PCR-F

TGGCTAATGGCTTTGCTTG

H11-PCR-R

TGGTTTTGAGTCCCCTTTCC

Cep112-PCR-F

CTGTGCCGTTTGGTTGAG

Cep112-PCR-R

CCACTCTCCCCGTTTTCAT

  1. 1The PAM sequences for sgRNAs are underlined
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BMC Biotechnology

ISSN: 1472-6750

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