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Fig. 3 | BMC Biotechnology

Fig. 3

From: Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

Fig. 3

Screening for an alternative electroporation buffer. (A) Microscopic photographs of electroporation of 13 kb Cas9-EGFP plasmid into PFFs with different buffers. T4: Cytoporation Medium T4; Ingenio: Ingenio® Electroporation Solution; Ent-E: Entranster-E; P3: Lonza 4D-Nucleofector™ X kit P3 buffer. Bar = 200 μm. The cells were electroporated with FF-113 + CA-137 dual-program and the photos were captured 24 h post-electroporation. (B) Comparison of transfection effects by delivering the 13 kb Cas9-EGFP plasmid using Entranster-E and P3 buffers in vessels. (C) Comparison of transfection effects by delivering the 13 kb Cas9-EGFP plasmid using Entranster-E and P3 buffers in strips. ns: P ≥ 0.05

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BMC Biotechnology

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