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Fig. 1 | BMC Biotechnology

Fig. 1

From: Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

Fig. 1

Electroporation of PFFs using Nucleocuvette™ vessels. The transfection efficiencies and cell survival rates after delivery of 10 µg Cas9-EGFP (A) and equimolar amount of pMAX-GFP (B) plasmid via the built-in programs of Lonza basic protocol. (C) Transfection efficiencies and cell survival rates after delivery of Cas9-EGFP plasmid via different dual-electroporation programs. (D) Representative microscopic photographs 24 h post electroporation. Bars = 200 μm. (E) Effect of plasmid dosage on transfection efficiency and cell survival rate. *P < 0.05, **P < 0.01

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BMC Biotechnology

ISSN: 1472-6750

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