Fig. 3
From: Development of a mammalian cell-based ZZ display system for IgG quantification
Cell sorting for stable CHO-ZZ cells. (A) Levels of ZZ display on the surface of the cells from the third-, fifth-, and seventh-round of sorting. (B) Percentage of ZZ-positive cells in the seventh round. (C) Identification of ZZ display by immunofluorescence imaging. (D) Time-course ZZ display of CHO-ZZ cells. The data was pooled from three independent experiments. CHO-ZZ cells were harvested, labelled with FITC-hIgG and then analysed by flow cytometry. For imaging, CHO-ZZ cells were seeded on the coverslips for two days. The cells were then fixed with 4% formaldehyde, labelled with FITC-hIgG and imaged by microscopy