Table 2 Composition of chemically defined medium for Paenibacillus polymyxa (Moppa medium) for main-cultures and its variations. The letters in brackets indicate which components were prepared together in a stock solution. In experiments with varying concentrations of medium components, the preparation of stock solutions was adapted according to the varying components
Ingredients | Â | Concentration in Moppa medium [g/L] | Concentration in supplemented Moppa medium [g/L] | Concentration in reduced Moppa medium [g/L] |
|---|---|---|---|---|
Main components | ||||
 Maltose syrup |  | 120 | 120 | 120 |
 Citric acid * H2O | (a) | 3.61 | 3.61 | 3.61 |
 K2HPO4 |  | 1 | 3 | 3 |
 (NH4)2SO4 | (a) | 1.07 | 3.21 | 3.21 |
 MgSO4 * 7 H2O |  | 1.62 | 1.62 | 1.62 |
 MES buffer |  | 19.5 | 39.0 | 39.0 |
 Antifoam |  | 0.2 | 0.2 | 0.2 |
 Ca(NO3)2 * 4 H2O |  | 0.342 | 0.342 | 0.342 |
Vitamins | ||||
 Ascorbic acid |  | 0.5 | 0.5 |  |
 Biotin |  | 0.00006 | 0.00006 | 0.00006 |
 Nicotinic acid | (b) | 0.0011 | 0.013 | 0.013 |
 Calcium pantothenate | (b) | 0.001 | 0.001 |  |
 p-aminobenzoic acid | (b) | 0.01 | 0.01 |  |
 Pyridoxamine 2*HCl | (b) | 0.006 | 0.006 |  |
 Pyridoxine HCl | (b) | 0.002 | 0.002 |  |
 Vitamin B12 | (b) | 0.001 | 0.001 |  |
 Thiamine HCl | (b) | 0.001 | 0.001 |  |
 Riboflavin |  | 0.001 | 0.001 |  |
 Orotic acid |  | 0.005 | 0.005 |  |
 Folic acid |  | 0.001 | 0.001 |  |
 Inositol |  | 0.0002 | 0.0002 |  |
Amino acids | ||||
 L-Alanine |  | 0.24 | 0.24 |  |
 L-Arginine |  | 0.125 | 0.125 | 0.125 |
 L-Aspartic acid |  | 0.42 | 0.42 |  |
 L-Cysteine |  | 0.13 | 0.13 |  |
 (S)-( +)-Glutamic acid |  | 0.5 | 0.5 | 0.5 |
 L-Glycine |  | 0.175 | 0.175 |  |
 L-Histidine |  | 0.15 | 0.15 | 0.15 |
 L-Isoleucine |  | 0.21 | 0.21 |  |
 L-Leucine |  | 0.475 | 0.475 |  |
 L-Lysine |  | 0.44 | 0.44 |  |
 L-Methionine |  | 0.2 | 0.2 | 0.2 |
 L-Phenylalanine |  | 0.275 | 0.275 |  |
 L-Proline |  | 0.675 | 0.675 | 0.675 |
 L-Serine |  | 0.34 | 0.34 |  |
 L-Threonine |  | 0.225 | 0.225 |  |
 L-Tryptophane |  | 0.05 | 0.05 |  |
 L-Tyrosine |  | 0.25 | 0.25 |  |
 L-Valine |  | 0.325 | 0.325 |  |
Nucleobases/ -sides | ||||
 Adenine |  | 0.01 | 0.01 |  |
 Guanine |  | 0.01 | 0.01 |  |
 Inosine |  | 0.005 | 0.005 |  |
 Xanthine |  | 0.01 | 0.01 |  |
 Thymidine |  | 0.005 | 0.005 |  |
 Uracil |  | 0.01 | 0.01 |  |
Trace elements | ||||
 ZnSO4 * 7 H2O | (c) | 0.009 | 0.009 | 0.009 |
 CoSO4 * 7 H2O | (c) | 0.004 | 0.004 | 0.004 |
 CuSO4 * 5 H2O | (c) | 0.004 | 0.004 | 0.004 |
 (NH4)6Mo7O24 * 4 H2O | (c) | 0.003 | 0.003 | 0.003 |
 CaCl2 * 2 H2O | (d) | 0.066 | 0.066 | 0.066 |
 MnCl2 | (d) | 0.016 | 0.016 | 0.016 |
 FeCl2 | (e) | 0.005 | 0.005 | 0.005 |
 FeCl3 * 6 H2O | (e) | 0.005 | 0.005 | 0.005 |