Fig. 5

Cultivation of Paenibacillus polymyxa with varying amino acid composition in microtiter plate. Supplemented Moppa medium (specified in Table 2) without vitamins (only with nicotinic acid and biotin) and with or without amino acid groups. nic.: nicotinic. Amino acid groups (gr.) are specified in Additional file 1: Table S2. Initial concentrations were: 54.1–56.3 g/L (a-d) and 54.1–56.1 g/L (e–h) maltose, 2.9–3.2 g/L (a-d) and 2.9–3.1 g/L (e–h) glucose, 3.0–3.1 g/L (a-d) and 3.0–3.1 g/L (e–h) citrate. a, e: Oxygen transfer rate (OTR), b, f: Final optical density (OD), total oxygen consumption (TOC) and pH, c, g: Final maltose, glucose and citrate concentration, d, h: Final acetoin and 2,3-butanediol concentration. a, b: For clarity, only every 20th measuring point over time is marked as a symbol. Mean values for OTR of at least three replicates with standard deviations as shadows are shown. b, f: TOC was determined based on OTR data of the replicates. Mean values for TOC of the replicates with standard deviations depicted as error bars are shown. b-h: For offline analysis, samples (wells) of the replicates of the OTR measurement were pooled at the end of the experiments. OD measurement of pooled samples was performed in triplicate and mean values with standard deviations depicted as error bars are shown. OD of pooled sample of the cultivation w/o amino acids was measured in duplicate and the mean value without standard deviation is shown. pH and concentrations of sugars and metabolites were determined in a single measurement of pooled samples. c, d, g: d.l. means that concentrations of components were lower than the detection limit. Final lactate concentrations were lower than the detection limit. Parameters in b-d and f–h were determined after 159.3 h. Cultivation conditions: temperature 33 °C, 48-round well plate, filling volume 0.8 mL, shaking frequency 1000 rpm, shaking diameter 3 mm