Fig. 4

Cultivation of Paenibacillus polymyxa only with growth relevant vitamins in microtiter plate. Supplemented Moppa medium (specified in Table 2) with or without vitamins (only with nicotinic acid and biotin). nic.: nicotinic. Initial concentrations were: 56.6–57.0 g/L maltose, 3.2–3.3 g/L glucose, 3.0 g/L citrate. a Oxygen transfer rate (OTR), b Final optical density (OD) and pH, c Final maltose and glucose concentration, d Final acetoin and 2,3-butanediol concentration. a For clarity, only every 18th measuring point over time is marked as a symbol. Mean values for OTR of four replicates with standard deviations as shadows are shown. Standard deviations are not well recognizable, because they are small. b-d For offline analysis, samples (wells) of the replicates of the OTR measurement were pooled at the end of the experiments. OD measurement of pooled samples was performed in triplicate and mean values with standard deviations depicted as error bars are shown. pH and concentrations of sugars and metabolites were determined in a single measurement of pooled samples. c d.l. means that concentrations of components were lower than the detection limit. Final citrate and lactate concentrations were lower than the detection limit. Parameters in b-d were determined after 133.3 h. Cultivation conditions: temperature 33 °C, 48-round well plate, filling volume 0.8 mL, shaking frequency 1000 rpm, shaking diameter 3 mm