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Fig. 6 | BMC Biotechnology

Fig. 6

From: Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate

Fig. 6

Influence of the Hpositionof black polymer rings on scattered light measurement during cultivation. Cultivation was performed with E. coli in Wilms-MOPS-medium with 20 g/L glucose using a 48-well microtiter plate (MTP-R48-B) in triplicates (n = 3). Polymer rings: ring without glucose crystals sprayed with black lacquer (Fig. 2C), dinner = 8 mm, Zheight = 3 mm, Hposition = 0.0 mm, 1.5 mm, 3.5 and 5.5 mm. As a control, wells without polymer rings were evaluated. Experimental parameters: initial pH = 7.5, VL = 0.8 mL, T = 37 °C, n = 1000 rpm, d0 = 3 mm, OD600 nm, start = 0.5. Commercial BioLector settings: Gain 15, λex,em = 620 nm, glass fibre in standard position (offset = 0 mm, Figure S3)

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