Fig. 3

Representative microscopic images after 5 days of rat kidney scaffold recellularization with human endothelial cells EA.hy 926. The left column displays multiple attached endothelial cells covering the vascular basement membrane as a monolayer after 0.66% SDS while after 3% SDS there was only a small number of detectable cells (right column). Evaluation of recellularization success was primarily obtained with HE-staining (A, B). Staining for CD-31 (C, D) confirms preservation of endothelial phenotype and staining for PCNA (E, F) indicates proliferative behavior. Apoptotic cells were detected by ISNT (G, H, arrows). Scale bar represents 100 μm. N = 3 for each column