Fig. 4

Ectopic expression of FOXP2 in chicken embryonic brain cells increases ELAVL4 and CNTNAP2 expression. A Structure of FOXP2-overexpressing plasmid. B FOXP2-overexpressing vector test using the in ovo electroporation and surrogate culture system. EGFP expression was analyzed by cNestin promoter in the embryonic brain at embryonic day 5 (E5). One day after electroporation, EGFP expression was observed by fluorescence microscopy in the injected region of the whole brain. Scale bar, 200 μm. EGFP expression was also observed by immunohistochemistry in paraffin sections using anti-GFP antibody. Scale bar, 200 μm (whole brain) and 50 μm (sectioned brain). C Quantitative RT-PCR analysis of FOXP2, CNTNAP2, and ELAVL4 genes in wild-type (WT) and FOXP2-overexpressing brain. Significant differences between groups were determined using Student’s t-tests. *p < 0.05, ****p < 0.0001. Embryos n = 4