Fig. 3
From: αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8
Delivery of EGFP into sEVs by MFG-E8 fusion expression. The sEVs from cells transfected with pCDNA3.4/EGFP or pCDNA3.4/MFG-E8-EGFP were analyzed by western blotting to confirm whether MFG-E8 could address other proteins into sEVs(a and Additional file 1: Figures S6, S7, S8). To identify the transfection efficiency of sEVs, the blank HEK293F cells were transfected with sEVs containing EGFP or MFG-E8-EGFP for 6 h and investigated under confocal microscopy (b)