Fig. 2
From: αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8
Isolation of sEVs and confirm the MFG-E8 is present outside the sEVs. sEVs were isolated from cell culture on day 4 after transient transfection of recombinant MFG-E8 and were analyzed by NTA analyzer (a), and western blotting (b and Additional file 1: Figures S4, S5) to confirm the existence of MFG-E8 in sEVs. Under the transmission electron microscopy, MFG-E8 was shown by anti-MFG-E8-AuNPs-mAb (black dots). Black dots, black arrows and red arrows refer to anti-MFG-E8-AuNPs-mAb, sEVs and MFG-E8, respectively.(c) (scale bar = 100 nm)