Fig. 1

a Schematic of the mutated Tyr locus. The target sequence (exon 2 of Tyr) recognized by crRNA1, 2, and 3 is shown in green. The PAM sequences are underlined. ssODN (containing wild-type nucleotide “G” that corresponds to mutated nucleotide “A”) is shown in orange below the target sequence. Nucleotide “A/T” marked in red is the mutation causative of the albino phenotype. Primers F and R used for amplification of the region spanning the target sequence are shown below Tyr. b Mid-gestational fetuses obtained from i-GONAD-treated females. Left: Albino fetus exhibiting depigmented eyes (red arrow). Right: Fetus exhibiting pigmented eyes (black arrow), reflecting successful KI of ssODN into the Tyr locus of albino rats. c Direct sequencing of PCR products derived from the albino (intact) fetus, KI fetus, KO fetus, and mosaic fetus