Fig. 4
From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12896-020-00608-z/MediaObjects/12896_2020_608_Fig4_HTML.png)
Phenotype of GD B. dothidea HTLW03 transformants. a Colony morphology of ΔBdo_05381–1, ΔBdo_05381–2, and ΔBdo_02540–1 in Petri plates containing PDA medium or PDA medium supplemented with 50 mg/L Congo red and 0.7 M NaCl. Accumulation of melanin in 6-day-old cultures is presented.b Comparison of the colony diameters of 60-h-old cultures on PDA medium. c Inhibitory effects of 50 mg/L Congo red and 0.7 M NaCl on the WT control and GD transformants. Bars represent the standard error of two independent experiments. The significance of the differences between the GD transformants (ΔBdo_05381–1, ΔBdo_05381–2, and ΔBdo_02540–1) and the WT control was analyzed based on an ANOVA and Duncan’s method. Different letters represent significant differences (P < 0.01)