Fig. 7

Myogenic differentiation of BMSC/Mb, ADSC/Mb, and C2C12 after long-term stimulation on PCL-collagen I-nanoscaffolds. Cells were stimulated with group 3 serum-free medium. Expressions are demonstrated in x-fold difference compared with BMSC/Mb and ADSC/Mb, stimulated with standard myogenic differentiation medium (control = 1) using the 2^-ΔΔCt-method. Markers are presented as mean ± standard deviation. MyHC2 (myosine heavy chain 2), ACTN2 (alpha actinin skeletal muscle 2), and MYOG (myogenin) were downregulated after 28 days of serum free myogenic differentiation for BMSC/Mb compared to controls. Statistical differences were tested with paired t-test or Wilcoxon test, as appropriate (n = 3). Level of significance was * p ≤ 0.05