Fig. 2

Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and CD29 and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes (b), osteocytes (c), and chondrocytes (d). Lipid vacuoles were visualized with oil red O staining (b), calcium deposits were stained with Alizarin Red S (c), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining (d). Inserts represent ADSC, cultured in proliferation medium as negative controls