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Fig. 3 | BMC Biotechnology

Fig. 3

From: Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber

Fig. 3

SDS-PAGE analysis of the induction and purification of GhKIS13A1 expressed in E.coli BL21. a, SDS-PAGE analysis of induction and purification of GhKIS13A1-His fusion protein. M: protein molecular marker; Lane 1: total proteins of cells after 2 h of IPTG induction; lane 2: total proteins of cells after 4 h of IPTG induction; lane 3: total proteins of cells after 6 h of IPTG induction; lane 4: total proteins of cells without IPTG induction; lane 5: purified GhKIS13A1-His fusion protein. b, GhKIS13A1-His fusion protein confirmed by western blot experiment with anti-6 × His antibody

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BMC Biotechnology

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