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Table 2 Kinetic parameters of WT 1593, WT sll0208 and some variants

From: Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

  

K m (mM)

k cat (min−1)

k cat /K m (min−1mM−1)

1593

WT [17

0.30 ± 0.02

0.48 ± 0.01

1.6 ± 0.2

L146T

0.34 ± 0.08

0.94 ± 0.08

2.76 ± 0.3

F150Y

0.20 ± 0.04

0.75 ± 0.04

3.75 ± 0.4

Q49H/F150Y

0.33 ± 0.05

0.87 ± 0.04

2.64 ± 0.2

Q49H/N123H/F150Y

0.35 ± 0.02

1.04 ± 0.02

2.97 ± 0.3

W178R

0.34 ± 0.08

1.81 ± 0.15

5.32 ± 0.4

sll0208

WT

0.35 ± 0.07

0.59 ± 0.04

1.69 ± 0.2

L148R

0.32 ± 0.08

0.91 ± 0.08

2.84 ± 0.3

D49H/N123H

0.38 ± 0.08

0.83 ± 0.08

2.18 ± 0.2

  1. The kinetic parameters against n-heptanal were determined
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BMC Biotechnology

ISSN: 1472-6750

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