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Fig. 2 | BMC Biotechnology

Fig. 2

From: Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

Fig. 2

The viability assays of cassava mesophyll protoplasts using the optimized protocol, i.e. preparation of mesophyll protoplasts in an enzyme solution (1.6% cellulase + 0.8% macerozyme) incubated at 25 °C (45 rpm) for 16 h in darkness. a Bright field image of protoplasts; (b) Protoplasts stained with FDA; (c) Merged image of protoplasts (Scale bar = 100 μm).

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BMC Biotechnology

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