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Table 1 Recipe for the modified artificial diet for M. separata

From: Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors

Component A

Component B

Corn flour

2–3 g

Glucose

1.8–2.4 g

Corn leaf flour

4–5 g

L-Ascorbic acid

0.3–0.4 g

Soybean flour

2.3–2.8 g

Distilled water

25 ml

Yeast extract

4–5 g

Component C

 

Casein

1–1.5 g

Distilled water

75 ml

Cholesterol

0.06–0.08 g

Agar

1.7–2 g

  

Sorbic acid

0.1–0.2 g

  1. Leaves of corn seedling were dehydrated with oven and were ground into powder using the pestle. Component A and B were mixed, then the component C was added