Table 1 Analysis of isolated antigen-specific B cells
Targeted antigen | Number of analyzed wells | Number of wells with HC gamma amplification | Number of wells with HC and LC associated (% recovery) | Mutations in variable HC and LC | Number of antigen-specific produced antibodies | Affinity determined by SPR (KD) |
|---|---|---|---|---|---|---|
β-galactosidase | 14 | 12 | 8 (57%) | Yes | 8 | 1 × 10−10 to 1 × 10−7M |
Fc-CD22 | 30 | 6 | 6 (20%) | Yes | 1 anti-CD22; 2 anti-Fc | 1,6 × 10−9M |
HLA-A | 7 | 6 | 6 (86%) | Yes | 1 | 1,27 × 10−8M |
HLA-A2/Pp65 | 24 | 11 | 9 (38%) | Yes | 3 | 6.10−8 to 1,4.10−7M |
CD22 | 72 | 49 | 44 (61%) | Yes | 27 | Not determined |