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Table 4 Effect of four factors on specific activity of soluble MnP

From: Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes

Experiment

A

B

C

D

Specific activity

Triton X-100

Tween-80

Glycerol

Ethanol

(U/mg)

(%)

(%)

(%)

(%)

 

1

1

1

1

1

0.411 ± 0.03

2

1

2

2

2

0.355 ± 0.02

3

1

3

3

3

0.361 ± 0.14

4

2

1

2

3

0.320 ± 0.03

5

2

2

3

1

0.185 ± 0.01

6

2

3

1

2

0.208 ± 0.07

7

3

1

3

2

0.167 ± 0.02

8

3

2

1

3

0.121 ± 0.03

9

3

3

2

1

0.252 ± 0.27

K1

1.127

0.897

0.741

0.849

 

K2

0.714

0.66

0.927

0.729

 

K3

0.54

0.822

0.714

0.801

 

k1

0.376

0.299

0.247

0.283

 

k2

0.238

0.22

0.309

0.243

 

k3

0.18

0.274

0.238

0.267

 

R

0.196

0.079

0.071

0.04

 
  1. Specific activity was obtained from the orthogonal design experiment. Each value is the average of three independent runs of experiment
  2. K1 represents the sum of the specific activity of each factor if the factor level is 1. K2 represents the sum of the specific activity of each factor if the factor level is 2, and so K3. k1 represents the average number of K1, and so on, for k2 and k3. R represents the difference of the largest and smallest k values of each factor