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Table 3 The optimal cryopreservation solutions containing HES identified for keratinocytes and fibroblasts

From: Cryopreservation of dermal fibroblasts and keratinocytes in hydroxyethyl starch–based cryoprotectants

Cell Type

Suspension

Monolayer

HaCaT

5% DMSO + 5% HES + 90% FCS or 10% HES + 90% FCS

5% DMSO + 5% HES + 90% FCS or 10% HES + 90% FCS

BJ

5% DMSO + 5% HES + 90% FCS or 10% HES + 90% FCS

5% DMSO + 5% HES + 90% FCS

Primary fibroblasts

5% DMSO + 5% HES + 90% FCS

N/A