Fig. 7

Cellular staining of primary fibroblast cells preserved as a monolayer. Representative immunofluorescence images are shown for cryopreserved primary fibroblast cells preserved as monolayers in different cryoprotective solutions. Three days after thawing, cells were stained for nuclei, actin, and mitochondria. For comparison, nuclei, actin, and mitochondria were stained in fresh primary fibroblast cells