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Fig. 1 | BMC Biotechnology

Fig. 1

From: A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum

Fig. 1

Growth of C. glutamicum strains on CGXII medium with 100 mM d-ribose under aerobic conditions. C. glutamicum strains were aerobically pre-cultivated in 50 mL of CGIII medium. After washing the cells in CGXII minimal medium, the cultures were inoculated to obtain optical densities at 600 nm of approximately 1.5 (dry cell weight of 0.45 g/L). When necessary, 0.5 mM IPTG was supplemented after inoculation. a WT, WT∆pyk1, WT∆pyk2 and WT∆pyk1∆pyk2. b WT∆pyk1/pXMJ19, WT∆pyk1/pXMJ19-pyk1 and WT∆pyk1/pXMJ19-pyk2. c WT∆pyk1∆pyk2/pXMJ19, WT∆pyk1∆pyk2/pXMJ19-pyk1 and WT∆pyk1∆pyk2/pXMJ19-pyk2. The data were derived from experiments performed at least in three times, and the error bars represent the standard deviations

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