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Fig. 6 | BMC Biotechnology

Fig. 6

From: Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

Fig. 6

Restoring EGFP expression using DNA methyltransferase (DNMT) inhibitor, 5-Aza-2′-deoxycitidine (5-Aza-dC), and histone deacetylase (HDAC) inhibitor, sodium butyrate (NaBu). Clones generated using CpGrich (B5, C4 unstable clones and C12 stable clone) and CpGfree (A1,B2 unstable clones and C5 stable clone) at week 8, the end of stability testing, were treated with DNMT or HDAC inhibitors for 24 h before MFI was measured again. Black bars represent samples without treatment, grey bars for samples treated with 5-Aza-dC and white bars represent samples treated with NaBu. Each bar represents duplicate measurements of two independent experiments for each clone. MFI values were normalized to the untreated control

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BMC Biotechnology

ISSN: 1472-6750

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