Fig. 4

Comparing relative EGFP gene copies and mRNA levels. EGFP Gene copies were determined for (a) CpGrich and (b) CpGfree (b) clones and relative EGFP mRNA levels for (c) CpGrich and (d) CpGfree clones at week 0 and week 8. β-actin and eukaryotic translation elongation factor-1 alpha-1 (EF-1α) served as the internal controls to normalize the variation in input amount and quality of DNA and mRNA respectively. Retained EGFP expression level, gene copy and mRNA values in the tables were calculated as the ratios of their values at the end of stability testing at week 8 to their starting levels at week 0. Black bar represents values for week 0 and the grey bar represents week 8. Each point and standard deviation represents the average derived from replicated measurements of two separately prepared samples