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Fig. 3 | BMC Biotechnology

Fig. 3

From: Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

Fig. 3

Comparing EGFP expressing level and stability of clones using the CpGrich and CpGfree promoters. a Mean fluorescence intensity (MFI) of 30 stably transfected clones for each promoter at week 0 before selection pressure was removed at the start of stability testing. Stability testing was subsequently performed by culturing the cells in selection free media for 8 weeks. The clones were randomly isolated from three separately generated pools. b Percentage of EGFP expressing cells after 8 weeks stability testing. c Retained EGFP expression after 8 weeks stability testing. Each point represents the MFI of an individual clone and the horizontal bar represents the average MFI of the 30 clones. Values of CpGfree clones which are statistically different from CpGrich are indicated by an *(p < 0.05)

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BMC Biotechnology

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