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Fig. 1 | BMC Biotechnology

Fig. 1

From: Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells

Fig. 1

Promoter sequences and schematic representation of the vector used in the study. a Sequence of the CpGrich (top strand) and CpGfree (bottom strand) promoters used in the study. Both promoters were cloned from vectors from InvivoGen. The promoters have 90 % similarity and alignment was done online using nucleotide BLAST at http://blast.ncbi.nlm.nih.gov/Blast.cgi. b Structure of the expression cassette used to express an enhanced green fluorescence protein (EGFP) and a mutant neomycin phosphotransferase selection marker with amino acid substitution D261G (mNPT)

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BMC Biotechnology

ISSN: 1472-6750

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