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Fig. 2 | BMC Biotechnology

Fig. 2

From: A novel set of vectors for Fur-controlled protein expression under iron deprivation in Escherichia coli

Fig. 2

Vector maps of pFCF1 and pFCF2. a Polypeptide sequence immediately downstream of the bidirectional promoter region found in gBlock1. Start codon sequence: black, FLAG sequence: green, TEV cleavage sequence: blue. MCS region from pUT18C colored by codons with restriction endonuclease sites shown below sequence. b pFCF1 vector map. Light blue bar indicates the promoter region. Unique restriction endonuclease sites shown in bold. FLAG: FLAG tag sequence; TEV: TEV cleavage site; TcR: tetracycline resistance gene; p15A ori: origin of replication. c pFCF2 vector map. Light blue bar indicates the promoter region. Unique restriction endonuclease sites shown in bold. HA: HA tag sequence; NeoR/KanR: neomycin/kanamycin resistance gene

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BMC Biotechnology

ISSN: 1472-6750

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