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Fig. 6 | BMC Biotechnology

Fig. 6

From: Application of surface plasmon resonance imaging to monitoring G protein-coupled receptor signaling and its modulation in a heterologous expression system

Fig. 6

A1R activation leads to suppression of mGluR1-mediated PKC activation. a and b In doxycycline-treated HEK293am cells, a 25-min application of DHPG (50 μM) increased R P/S . A 10-min pre-conditioning with the A1R antagonist R-PIA (500 nM) abolished the DHPG (50 μM)-induced R P/S increase. R-PIA continued to be present in the saline throughout the measurement. The data were obtained from cultures different from those in Fig. 5. a Sample images of the reflection of the P-polarized light. b Mean time-plots of R P/S . Error bars, ±SEM. ***, p < 0.0001 at 25 min of the DHPG application onset (van der Waerden rank sum test). Plot with gray triangles, the response of the HEK293gm cells treated with doxycycline and pre-conditioned with R-PIA (5 μM)

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BMC Biotechnology

ISSN: 1472-6750

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