Fig. 5

SPR imaging can detect PKC translocation initiated by ligand binding to mGluR1. a and b In doxycycline-treated HEK293am cells, the mGluR1 agonist DHPG (50 μM) increased R _P/S and this effect of DHPG was abolished with a 10-min pre-conditioning with the PKC inhibitor BIM1 (300 nM). BIM1 continued to be present in the saline throughout the measurement. a Sample images of the reflection of the P-polarized light. b Mean time-plots of R _P/S . Error bars, ±SEM. ***, p < 0.0001 at 25 min of the DHPG application onset (van der Waerden rank sum test). c and d Dose-dependence of DHPG-induced R _P/S increase. c Sample mean response of HEK293am cells to serial applications of a test dose (50 μM) and a reference dose (5 mM) of DHPG. On such a mean time-plot, the extents of quasi-maximal R _P/S increases with the test and reference doses (a and b, respectively) were measured. d Dose-response relation depicted using the ratio a/b. Each dot indicates the data obtained from the mean response of 17-42 ROIs. Sigmoid curve, Hill function fitted to the data