Fig. 1From: HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host speciesGeneral workflow of the HybriFree method. The key steps include (STEP 1) the enrichment of source material by capturing cells producing specific antibodies; (STEP 2) the amplification of VH and VL cDNA; (STEP 3) construction of a combinatorial VH-VL library in scFv-Fc (or IgG) expression plasmid DNA format; and (STEP 4) screening proper VH-VL combinations after expression of MAbs in mammalian cellsBack to article page