pdFVII | CHOrFVII | HEK293rFVII | BHKrFVII | |
---|---|---|---|---|
rFVII specific activity calculated from PT clotting values per mg protein (antigen) [1000 IU/mg] | Not done, theoretical specific activity 2.0 kIU/mg | 2.0 | 2.3 | 1.8 |
Peptide map | Similar | |||
γ-carboxylation of E35; i.e. abundance of 10th Gla [%] | 82 % | 53 % | 56 % | 53 % |
Presence of propeptide | Not detected by peptide mapping | |||
Hydroxylation of D68 [%] | <1 % | 9 % | 7 % | 15 % |
O-glycosylation on S52 / S60; only three major identified types shown [%] | 31 %: Glc(Xyl)2 / Fuc | 56 %: Glc(Xyl)2 / Fuc | 62 %: Glc(Xyl)2 / Fuc | 21 %: Glc(Xyl)2 / Fuc |
27 %: GlcXyl / Fuc | 7 %: GlcXyl2 | 7 %: GlcXyl / Fuc | 50 %: Glc / Fuc | |
15 %: Glc / Fuc | 14 %: Glc / Fuc | 10 %: Glc / Fuc | 12 %: Glc / - |