Fig. 4
From: Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
Electron micrographs of negatively-stained CCMV-CP/ NΔ24-CP loop insertion and fusion constructs expressed in E. coli. (a) Reassembled VLP pellet of βF-βG-His (A1), βF-βG-M2e(7) (A2), βF-βG-M2e(15) (A3) and βH-βI-M2e(23) (A4). (b) CCMV virions purified from cowpea plants (B1), CCMV-CP VLPs (B2), immuno-gold labeled CCMV-CP (B3), His-CCMV (B4), CCMV-His (B5), His-CCMV-His (B6), NΔ24-CP (B7), His-NΔ24-CP (B8), M2e(23)NΔ24-CP (B9), His-HA-NΔ24-CP (B10), HA-NΔ24-CP (B11), VP1-CP (B12), 2C-CP (B13), VP1-NΔ24-CP (B14), 2C-NΔ24-CP (B15), CP-HA (B16), CP-M2e(23) (B17, B18).(c) Higher resolution of four selected CCMV particles including CCMV virion, CCMV-CP, NΔ24-CP and VP1-NΔ24-CP shown on the top-right of each electron micrograph. Magnification 20,000x, scale bar represents 200 nm