Figure 1

The map for the expression plasmid pKLAC2- xynZG in K. lactis . xynZG (the target gene encoding XYNZG), P_LAC4-PBI (LAC4 Promoter), α-MF (α-mating factor secretion leader sequence), Ap^R (Ampicillin resistance gene), amdS (encoding acetamidase to break down acetamide for use as a nitrogen source), ori (origin of E. coli), TT_LAC4 (LAC4 transcription terminator).