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Table 5 Fold-change data comparison between different microarray labeling methods and qPCR for selected genes.

From: A rapid and inexpensive labeling method for microarray gene expression analysis

 

Fold-change

Gene

Microarrays

 
 

Direct Random

Indirect

ds-cDNA

qPCR

YNL072W (RNH201; Ref1)

1.0

1.0

1.0

1.0

YLR185W (RPL37a; Ref2)

1.0

1.1

0.9

1.0

YBL005W (PDR3)

2.2**

1.9

2.1

1.6**

YDR011W (SNQ2)

3.4**

4.2*

3.0*

4.6**

YDL020C (RPN4)

1.9**

1.8*

1.7

3.6**

YFR003C (YPI1)

1.0

1.1

0.8**

1.0

YGR035C

12.6**

11.7**

7.3**

10.8**

YGR052W (FMP48)

3.2**

3.0*

3.2*

2.9**

YGR236C (SPG1)

0.4**

0.4*

0.7*

0.5**

YLR346C

7.7**

6.9**

2.0**

6.2**

YOL156W

12.9**

9.7**

4.2**

6.8**

  1. (* p-value < 0.05; ** p-value < 0.01)