Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens

BMC Biotechnology

Table 2 Invasion inhibition activity and antigen specificity of the selected scFvs.

scFv	Inhibition of invasion (%)¹	Binding to complex antigens²			Putative mol. weight of recognized antigen (kDa)³		Sporozoite surface binding⁴
		Oocyst extract	Sporozoite extract	Merozoite extract	Reduced	Non-reduced	FACS	IFAT
AA19	28 ± 14	+	-	-	83-175	83-175	-	-
AA28	36 ± 16	+	-	-	100-150	100-150	-	-
AB09	52 ± 15	+	+	-	n.d.	15-20	+	+
AB21	32 ± 7	+	+	-	n.d.	15-20	+	+
AB28	25 ± 3	+	+	-	Negative	15-20	+	+
AD10	36 ± 12	+	+	-	Negative	15-20	+	+

¹ In each experiment, 10 μg bacterially expressed and purified scFv was used. Means and SDs of triplicates are shown.
² As determined by ELISA.
³ As estimated by comparison with molecular weight markers in Western blot analyses.
⁴ As determined by IFAT and flow cytometry (FACS).
+, binding; -, no binding; n.d., not determined.

ISSN: 1472-6750