Table 2 Results of affinity selections with different targets
From: A compact phage display human scFv library for selection of antibodies to a wide variety of antigens
Antigens | Round of panning | Number of clone after 1st round | Number of binding phage after 1st round | Number of binding phage after 2nd round | Number of soluble scFv fragments | Number of different scFv producing clone |
|---|---|---|---|---|---|---|
Aflatoxin | 1 | 2.5 × 102 | 4/56 | - | 3/4 | 3/3 |
Attenuated Rabies viruses | 2 | 1.2 × 103 | 1/96 | 7/96 | 8/8 | 2/8 |
Amylase | 1 | 8.8 × 10 | 3/88 | - | 3/3 | NA |
BSA | 1 | 2.0 × 103 | 6/96 | - | NA | NA |
Cobra snake venom | 2 | 1.4 × 104 | 1/96 | 9/96 | 3/3 | 1/10 |
Cholangiocarcinoma cell KKU-100 | 1 | 2.2 × 103 | 2/96 | - | NA | 2/2 |
Green pit viper venom | 2 | 5.1 × 104 | 0/96 | 0/96 | NA | NA |
GST | 1 | 4.3 × 102 | 6/28 | - | NA | NA |